Journal: Genes

Article Title: Association of Interleukin Genes IL10 and IL10RB with Parameters of Overweight in Military Students

doi: 10.3390/genes13020291

Figure Lengend Snippet: The characteristics of single nucleotide polymorphisms for IL10 and IL10RB genes.

Article Snippet: All samples were genotyped using TaqMan ® Pre-Designed SNP Genotyping Assays for IL10 (rs1518110) C___8828802_20, IL10 (rs3024491) C__15983669_10, IL10RB (rs2834167) C__25473700_10 single-nucleotide polymorphisms (SNPs) (Applied Biosystems, USA) on a CFX Connect Real-Time PCR Detection System (BioRad, USA) according to the manufacturer’s instructions and recommendations.

Techniques: Functional Assay, Variant Assay

Journal: Genes

Article Title: Association of Interleukin Genes IL10 and IL10RB with Parameters of Overweight in Military Students

doi: 10.3390/genes13020291

Figure Lengend Snippet: The genotype frequencies from Hardy-Weinberg expectations and minor allele frequencies (MAF).

Article Snippet: All samples were genotyped using TaqMan ® Pre-Designed SNP Genotyping Assays for IL10 (rs1518110) C___8828802_20, IL10 (rs3024491) C__15983669_10, IL10RB (rs2834167) C__25473700_10 single-nucleotide polymorphisms (SNPs) (Applied Biosystems, USA) on a CFX Connect Real-Time PCR Detection System (BioRad, USA) according to the manufacturer’s instructions and recommendations.

Techniques:

Journal: Genes

Article Title: Association of Interleukin Genes IL10 and IL10RB with Parameters of Overweight in Military Students

doi: 10.3390/genes13020291

Figure Lengend Snippet: Association analysis of the IL10 rs3024491 polymorphism with BMI.

Article Snippet: All samples were genotyped using TaqMan ® Pre-Designed SNP Genotyping Assays for IL10 (rs1518110) C___8828802_20, IL10 (rs3024491) C__15983669_10, IL10RB (rs2834167) C__25473700_10 single-nucleotide polymorphisms (SNPs) (Applied Biosystems, USA) on a CFX Connect Real-Time PCR Detection System (BioRad, USA) according to the manufacturer’s instructions and recommendations.

Techniques:

Journal: Genes

Article Title: Association of Interleukin Genes IL10 and IL10RB with Parameters of Overweight in Military Students

doi: 10.3390/genes13020291

Figure Lengend Snippet: Association analysis of the IL10 rs1518110 polymorphism with BMI.

Article Snippet: All samples were genotyped using TaqMan ® Pre-Designed SNP Genotyping Assays for IL10 (rs1518110) C___8828802_20, IL10 (rs3024491) C__15983669_10, IL10RB (rs2834167) C__25473700_10 single-nucleotide polymorphisms (SNPs) (Applied Biosystems, USA) on a CFX Connect Real-Time PCR Detection System (BioRad, USA) according to the manufacturer’s instructions and recommendations.

Techniques:

Journal: Genes

Article Title: Association of Interleukin Genes IL10 and IL10RB with Parameters of Overweight in Military Students

doi: 10.3390/genes13020291

Figure Lengend Snippet: Haplotype-based association of IL10 rs3024491 and IL10 rs1518110 with BMI, Fat%, FMI.

Article Snippet: All samples were genotyped using TaqMan ® Pre-Designed SNP Genotyping Assays for IL10 (rs1518110) C___8828802_20, IL10 (rs3024491) C__15983669_10, IL10RB (rs2834167) C__25473700_10 single-nucleotide polymorphisms (SNPs) (Applied Biosystems, USA) on a CFX Connect Real-Time PCR Detection System (BioRad, USA) according to the manufacturer’s instructions and recommendations.

Techniques:

Journal: Genes

Article Title: Association of Interleukin Genes IL10 and IL10RB with Parameters of Overweight in Military Students

doi: 10.3390/genes13020291

Figure Lengend Snippet: Association analysis of the IL10RB rs2834167 × IL10 rs1518110 interaction with Fat% (codominant model).

Article Snippet: All samples were genotyped using TaqMan ® Pre-Designed SNP Genotyping Assays for IL10 (rs1518110) C___8828802_20, IL10 (rs3024491) C__15983669_10, IL10RB (rs2834167) C__25473700_10 single-nucleotide polymorphisms (SNPs) (Applied Biosystems, USA) on a CFX Connect Real-Time PCR Detection System (BioRad, USA) according to the manufacturer’s instructions and recommendations.

Techniques:

Journal: Genes

Article Title: Association of Interleukin Genes IL10 and IL10RB with Parameters of Overweight in Military Students

doi: 10.3390/genes13020291

Figure Lengend Snippet: Association analysis of the IL10RB rs2834167 × IL10 rs1518110 interaction with Fat% (dominant model).

Article Snippet: All samples were genotyped using TaqMan ® Pre-Designed SNP Genotyping Assays for IL10 (rs1518110) C___8828802_20, IL10 (rs3024491) C__15983669_10, IL10RB (rs2834167) C__25473700_10 single-nucleotide polymorphisms (SNPs) (Applied Biosystems, USA) on a CFX Connect Real-Time PCR Detection System (BioRad, USA) according to the manufacturer’s instructions and recommendations.

Techniques:

Journal: Genes

Article Title: Association of Interleukin Genes IL10 and IL10RB with Parameters of Overweight in Military Students

doi: 10.3390/genes13020291

Figure Lengend Snippet: Percentage of genotype IL10 (rs1518110) and IL10RB (rs2834167) with Fat%.

Article Snippet: All samples were genotyped using TaqMan ® Pre-Designed SNP Genotyping Assays for IL10 (rs1518110) C___8828802_20, IL10 (rs3024491) C__15983669_10, IL10RB (rs2834167) C__25473700_10 single-nucleotide polymorphisms (SNPs) (Applied Biosystems, USA) on a CFX Connect Real-Time PCR Detection System (BioRad, USA) according to the manufacturer’s instructions and recommendations.

Techniques:

Journal: Genes

Article Title: Association of Interleukin Genes IL10 and IL10RB with Parameters of Overweight in Military Students

doi: 10.3390/genes13020291

Figure Lengend Snippet: Percentage of genotype IL10 (rs1518110) and IL10RB (rs2834167) with Fat%.

Article Snippet: All samples were genotyped using TaqMan ® Pre-Designed SNP Genotyping Assays for IL10 (rs1518110) C___8828802_20, IL10 (rs3024491) C__15983669_10, IL10RB (rs2834167) C__25473700_10 single-nucleotide polymorphisms (SNPs) (Applied Biosystems, USA) on a CFX Connect Real-Time PCR Detection System (BioRad, USA) according to the manufacturer’s instructions and recommendations.

Techniques:

Journal: Pharmacogenetics and genomics

Article Title: The functional G143E variant of carboxylesterase 1 is associated with increased clopidogrel active metabolite levels and greater clopidogrel response

doi: 10.1097/FPC.0b013e32835aa8a2

Figure Lengend Snippet: Schematic of the major enzymatic steps in clopidogrel metabolism. CES1, carboxylesterase 1; CYP, cytochrome P450.

Article Snippet: CES1 G143E (rs71647871) SNP genotyping was performed using a TaqMan SNP genotyping assay (Applied Biosystems, Foster City, California, USA).

Techniques:

Journal: Pharmacogenetics and genomics

Article Title: The functional G143E variant of carboxylesterase 1 is associated with increased clopidogrel active metabolite levels and greater clopidogrel response

doi: 10.1097/FPC.0b013e32835aa8a2

Figure Lengend Snippet: Association of the carboxylesterase 1 (CES1) G143E variant (rs71647871) with ADP-stimulated platelet aggregation before (a, b) and after (c, d) clopidogrel administration in participants of the Pharmacogenomics of Anti-Platelet Intervention (PAPI) study (a, c) and coronary intervention patients at Sinai Hospital of Baltimore (b, d).

Article Snippet: CES1 G143E (rs71647871) SNP genotyping was performed using a TaqMan SNP genotyping assay (Applied Biosystems, Foster City, California, USA).

Techniques: Variant Assay

Journal: Pharmacogenetics and genomics

Article Title: The functional G143E variant of carboxylesterase 1 is associated with increased clopidogrel active metabolite levels and greater clopidogrel response

doi: 10.1097/FPC.0b013e32835aa8a2

Figure Lengend Snippet: Effect of CES1 143E, CYP2C19 *2, and CYP2C19 *17 variants on postclopidogrel ADP-stimulated platelet aggregation in participants of the Amish Pharmacogenomics of Anti-Platelet Intervention study

Article Snippet: CES1 G143E (rs71647871) SNP genotyping was performed using a TaqMan SNP genotyping assay (Applied Biosystems, Foster City, California, USA).

Techniques:

Journal: Horticulture Research

Article Title: Varietal identification of tea ( Camellia sinensis ) using nanofluidic array of single nucleotide polymorphism (SNP) markers

doi: 10.1038/hortres.2014.35

Figure Lengend Snippet: Call map view from the dynamic array IFC, displaying SNP fingerprints of genotyped tea varieties, shows the computer generated image of the genotype calls for each of the individual reaction chambers. Each column (vertical direction) represents data from one assay that correlated to the SNP genotyping assay loaded from each assay inlet.

Article Snippet: The Fluidigm SNPtype TM Genotyping Reagent Kit was used according to the manufacturer’s instructions.

Techniques: Generated, SNP Genotyping Assay

Journal: Experimental Hematology

Article Title: Determination of complex subclonal structures of hematological malignancies by multiplexed genotyping of blood progenitor colonies

doi: 10.1016/j.exphem.2017.09.011

Figure Lengend Snippet: Multiplexed SNP genotyping is an efficient method for genotyping BFU-E colonies . Number of colonies for which a genotype could be called out of 96 colonies for each mutation is shown. Five genes were genotyped by capillary sequencing and multiplexed SNP genotyping. Shaded regions highlight the number of colonies for which the same genotype was called by both technologies.

Article Snippet: The diluted amplified product was loaded onto the 192.24 Dynamic Array IFC for SNP Genotyping (BMK-M-192.24GT, Fluidigm) alongside a sample premixture including ROX reference dye and real-time master mixture.

Techniques: Mutagenesis, Sequencing

Journal: Molecular Psychiatry

Article Title: Molecular evidence of N -methyl- D -aspartate receptor hypofunction in schizophrenia

doi: 10.1038/mp.2012.137

Figure Lengend Snippet: NR1 expression is reduced in people with schizophrenia. Dorsolateral prefrontal cortex (DLPFC) NR1 mRNA expression, measured by quantitative reverse transcription-PCR (qRT-PCR) and normalized to the geometric mean of four housekeeping genes, was reduced in people with schizophrenia relative to normal controls ( a ). NR1 protein expression, normalized to alpha-tubulin, was quantified by western blot (representative blot shown in ( b ). A single band for NR1 was present at the predicted size (∼120 kDa). NR1 protein was reduced in the DLPFC of people with schizophrenia compared with matched controls ( c ). ** P ⩽0.01, *** P <0.005. C, control; S, schizophrenia case.

Article Snippet: The NR2B single-nucleotide polymorphisms (SNPs) rs1805502 (T5988C 3′UTR), rs1805247 (T4197C), rs7301328 (C366G exon 2) and rs1019385 (T200G) and NR1 SNP rs11146020 (G1001C) genotypes were determined using TaqMan SNP genotyping assays (Applied Biosystems) (see ), with a call rate of 97.9%.

Techniques: Expressing, Reverse Transcription, Quantitative RT-PCR, Western Blot, Control

Journal: Molecular Psychiatry

Article Title: Molecular evidence of N -methyl- D -aspartate receptor hypofunction in schizophrenia

doi: 10.1038/mp.2012.137

Figure Lengend Snippet: Effect of NR2B SNP rs1805502 on reasoning ability, NR1 mRNA and protein in controls and schizophrenia. NR2B rs1805502 genotype is related to reasoning ability (WAIS-III Arithmetic scores) in people with schizophrenia ( a ). Schizophrenia carriers of the C allele of rs1805502 display a significantly worse performance than homozygous (TT genotype) people with schizophrenia. Minor allele carriers with schizophrenia also perform worse than control carriers of the minor allele. NR1 mRNA expression ( b ) and protein levels ( c ) are significantly lower in individuals with schizophrenia who were C allele carriers than in control individuals. * P <0.05, ** P <0.01 and *** P <0.0001.

Article Snippet: The NR2B single-nucleotide polymorphisms (SNPs) rs1805502 (T5988C 3′UTR), rs1805247 (T4197C), rs7301328 (C366G exon 2) and rs1019385 (T200G) and NR1 SNP rs11146020 (G1001C) genotypes were determined using TaqMan SNP genotyping assays (Applied Biosystems) (see ), with a call rate of 97.9%.

Techniques: Control, Expressing

Journal: Pharmacogenetics and genomics

Article Title: cis -acting regulatory elements regulating CYP3A4 transcription in human liver

doi: 10.1097/FPC.0000000000000402

Figure Lengend Snippet: Panel a. Reporter gene assays showing luciferase activities of R4 harboring either the reference (G) or variant (A) allele of rs62471956. Mean ± SD, compared to pGL3 vector, *p<0.05, ***p<0.001; compared to reference allele (Ref), ###p<0.001. Panel b & c. Adjusted mRNA levels of CYP3A4 (b) or CYP3A43 (c) in 136 human liver samples (log 10 scale) grouped by rs62471956 genotype. Statistical analyses were performed using a multiple linear regression model. Box and horizontal lines: 25th to 75th percentiles and mean; whiskers: minimum and maximum values.

Article Snippet: Genotyping: SNP rs62471956 was genotyped using the OpenArray genotyping platform (probe# ANH6F7J, QuanStudio 12K Flex System) according to the manufacturer’s protocol (Life Technologies, California, USA).

Techniques: Luciferase, Variant Assay, Plasmid Preparation

Journal: Pharmacogenetics and genomics

Article Title: cis -acting regulatory elements regulating CYP3A4 transcription in human liver

doi: 10.1097/FPC.0000000000000402

Figure Lengend Snippet: Schematic diagram of the competitive interactions between R2 and R4 with the CYP3A4 promoter and changes in gene expression under different conditions: typical conformation (a), R4 deletion (b) and in the presence of the rs62471956 A allele (c). The thickness of the lines indicate the strength of the interactions, arrows located next to CYP3A4 and CYP3A43 indicate increased (upward) or decreased (downward) gene expression compared to the normal condition..

Article Snippet: Genotyping: SNP rs62471956 was genotyped using the OpenArray genotyping platform (probe# ANH6F7J, QuanStudio 12K Flex System) according to the manufacturer’s protocol (Life Technologies, California, USA).

Techniques: Gene Expression

Journal: Brazilian Journal of Psychiatry

Article Title: Cannabinoid receptor gene polymorphisms and cognitive performance in patients with schizophrenia and controls

doi: 10.1590/1516-4446-2020-1650

Figure Lengend Snippet: Allelic and genotypic frequencies of CNR1 and CNR2 gene polymorphisms

Article Snippet: Genotyping of the cannabinoid receptor polymorphisms was performed using a 5' nuclease TaqMan allelic discrimination assay: TaqMan ® SNP Genotyping assay rs12720071 (for CNR1 ) and rs2229579 (for CNR2 ) from Applied Biosystems (by Life Technologies, Carlsbad, CA, USA).

Techniques:

Journal: Brazilian Journal of Psychiatry

Article Title: Cannabinoid receptor gene polymorphisms and cognitive performance in patients with schizophrenia and controls

doi: 10.1590/1516-4446-2020-1650

Figure Lengend Snippet: Cannabinoid receptor 1 ( CNR1 ) rs12720071 polymorphism genotype and cognitive performance in patients with schizophrenia

Article Snippet: Genotyping of the cannabinoid receptor polymorphisms was performed using a 5' nuclease TaqMan allelic discrimination assay: TaqMan ® SNP Genotyping assay rs12720071 (for CNR1 ) and rs2229579 (for CNR2 ) from Applied Biosystems (by Life Technologies, Carlsbad, CA, USA).

Techniques: